GSM5776709: krrA 205-3; Bacillus anthracis; RNA-Seq - SRA

SRX13653995: GSM5776709: krrA 205-3; Bacillus anthracis; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 26.6M spots, 8G bases, 2.5Gb downloads

External Id: GSM5776709_r1

Submitted by: VUMC

Study: A Bacillus anthracis RNA binding protein post-transcriptionally regulates two component signaling through RNA turnover [RNA-Seq]

PRJNA795439 • SRP353949 • All experiments • All runs

show Abstracthide Abstract

To uncover the effects of KrrA regulation on gene transcription and define the bacterial response imposed by this regulation, a transcriptomic study was carried out in which Bacillus anthracis ?krrA was compared to WT in two growth conditions: LB in the presence or absence of '205. Overall design: Two strains of Bacillus anthracis (WT and krrA deletion mutant) in the presence or absence of 50 µM '205 were compared. RNA was isolated from each of the strains and conditions in triplicte and RNA sequencing performed. Transcriptomes and changes in expression patters were compared across each samples using CLC genomics workbench.

Sample: krrA 205-3

SAMN24720674 • SRS11539983 • All experiments • All runs

Organism: Bacillus anthracis

Library:

Name: GSM5776709

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total bacterial RNA was isolated utilizing the Qiagen RNeasy mini kit. DNA was removed by treatment with Invitrogen TURBO Dna-free kit and rRNA was depleted using the Invitrogen RiboMinus Transcriptome Isolation kit (bacteria) and the RiboMinus Concentration Module Library construction was performed using NEBNext Ultra II RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina

Runs: 1 run, 26.6M spots, 8G bases, 2.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR17483471	26,611,364	8G	2.5Gb	2022-01-10

ID:: 18989545

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